A New Method for the Determination of Serum Iron: Potentiostatic Coulometry with the Ferrochem
نویسندگان
چکیده
The well-known methods for the determination of serum iron, which are based on colour reactions with bathophenanthroline disulfonate, Ferrozine® or Chromazurol B, require — even on a microscale — sample amounts between 0.1 and 1.0 nil of serum (1,2,3). These sample volumes are too large for paediatric purposes. Reduction of sample size is possible in flame atomic absorption spectrophotometry using an injection technique (4) or in flameless atomic absorption spectrophotometry and X-ray fluorescence analysis (5), but both methods are not feasible for routine laboratories. A new analyser, Ferrochem 3050, developed by Environmental Sciences Associates (ESA) is based on potentiostatic coulometry and requires only a 50 μΐ sample for serum iron determination. In addition, this analyser allows the determination of total iron binding capacity. The present study evaluates precision, accuracy and practicability of this novel method in regard to the needs of a routine laborar tory.
منابع مشابه
A commercial electrochemical method evaluated for measurement of iron status.
I evaluated a commercial electrochemical method (Environmental Science Associates), based on potentiostatic coulometry, for determining iron and total iron-binding capacity (TIBC). The method requires 25 and 100 microL of serum, respectively, for these measurements. Serum iron concentrations so determined in 99 normal volunteers satisfactorily correlated with those determined by the Iron Panel ...
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